Neogen – Allergen Testing
Designed for your lab
Simplify workflow and be confident in your allergen testing process. Neogen™ Allergen Testing Products were designed with your needs in mind by a team of experts who use these tests every day. Based on extensive experience with immunoassay technology and testing thousands of unique food samples, they set out to design tests to detect both processed and unprocessed proteins with a simplified workflow. It took years of careful antibody selection and formulation development to create the simplicity and performance provided by these kits. Supported by Neogen scientists and engineers, these test kits give you the reliability and efficiency you have come to expect from Neogen.
Designed to detect processed and unprocessed proteins
With more variety than ever, today’s food products often require complex processing, which can alter the nature of allergenic proteins in food, making them harder to detect. Neogen™ Allergen Testing Products are designed to overcome this challenge by detecting both processed and unprocessed proteins for more predictable, accurate results in your lab.
Versatility across sample types
Neogen™ Allergen Testing Products can be used on environmental swabs, clean-inplace (CIP) final rinse water, ingredients and food samples.
Available Models
The Neogen™ Allergen Protein Rapid Kits are intended for screening for the presence of specific proteins in clean-in-place (CIP) final rinse water, environmental swab samples, food ingredients and processed food products.
The Neogen Allergen Protein Rapid Kits utilize a lateral flow device (LFD) that is an immunochromatographic test method utilizing antibodies which are specific for the detection of proteins from specific allergenic foods. Positive results are visualized by the presence of three lines: a control line, a hook line and a test line, when the target protein is present in a defined range of concentration. These limits will vary by a specific protein assay, and the limit of detection may vary depending on the matrix tested. Samples containing high amounts of target protein in a sample may result in an invalid test which will result in the absence of the hook line and/or test line.
- Results visible at 11 ± 1 minute after application of the sample.
- Rapid, qualitative immunochromatographic lateral flow test cartridges designed for the detection of allergenic food proteins.
- Designed to detect both processed and unprocessed allergen proteins.
- Test can accommodate environmental swab, CIP rinse water and food samples.
- Neogen™ Environmental Collection Swabs and Neogen™ Transfer Pipettes sold separately.
Lateral Flow Devices for Specific Allergen Detection
Streamline your process and simplify your allergen testing with Neogen™ Allergen Protein Rapid Tests — qualitative immunochromatographic assays for rapid in-plant monitoring of specific food allergens. Designed for accurate detection of processed and unprocessed allergen proteins, these fast, easy tests can be used for clean-in-place (CIP) final rinse water, environmental swab samples, raw ingredients and finished food products. We’ve taken the hassle out of allergen testing. You see the results.
Lateral Flow Rapid Kits
| Allergen Proteins | Catalog Number | Assay Time | Limit of Detection |
| Almond | L25ALM | 11 min ± 1 min | 2.0 ppm |
| Milk | L25MLK | 11 min ± 1 min | 3.0 ppm |
| Cashew | L25CHW | 11 min ± 1 min | 2.0 ppm |
| Coconut | L25COC | 11 min ± 1 min | 2.0 ppm |
| Egg White | L25EGG | 11 min ± 1 min | 0.5 ppm |
| Fish | L25FSH | 11 min ± 1 min | 1.0 ppm |
| Gluten | L25GLU | 11 min ± 1 min | 5.0 ppm |
| Hazelnut | L25HZL | 11 min ± 1 min | 2.0 ppm |
| Peanut | L25PNT | 11 min ± 1 min | 1.0 ppm |
| Pecan | L25PEC | 11 min ± 1 min | 3.0 ppm |
| Pistachio | L25PST | 11 min ± 1 min | 2.0 ppm |
| Soy | L25SOY | 11 min ± 1 min | 2.0 ppm |
| Walnut | L25WAL | 11 min ± 1 min | 2.0 ppm |
All kits include 25 lateral flow devices.
Neogen™ Allergen Protein Enzyme-Linked Immunosorbent Assay (ELISA) Tests
Get confirmed, quantifiable results with solid phase sandwich ELISA tests for monitoring specific food allergens across a wide variety of foods. Designed for accurate detection of processed and unprocessed target allergen proteins, this broad portfolio of ELISA assays is one of the most comprehensive available, and can be used for clean-in-place (CIP) final rinse water, environmental swab samples, food ingredients and processed food products. A universal extraction protocol streamlines testing for multiple analytes, saving you reagents and valuable time. It’s the simple way to master allergen testing.
The Neogen™ Allergen Protein ELISA Kits are intended for the detection of specific proteins in clean-in-place (CIP) final rinse water, environmental swab samples, food ingredients and processed food products.
The Neogen Allergen Protein ELISA Kits utilize a sandwich ELISA. The target protein present in the sample react with the specific antibodies, which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, antibodies conjugated with horseradish peroxidase (HRP) are added. This enzyme-labeled antibody forms complexes with the previously bound target protein. Following a second washing step, the enzyme bound to the immunosorbent is detected by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The color development from this enzymatic reaction varies directly with the concentration of target protein in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of the target protein in the test sample. The quantity of protein in the test sample can be extrapolated from the standard curve, constructed from standards of known concentration, and adjusted to consider the sample dilution.
- Designed to detect both processed and unprocessed target allergen proteins.
- Test can accommodate environmental swab, CIP rinse water and food samples.
- A universal extraction protocol streamlines testing for multiple analytes, saving you reagents and valuable time.
- Neogen™ Environmental Collection Swabs sold separately.
Elisa Test
| Allergen Proteins | Catalog Number | Assay Time | Limit of Detection | Limit of Quantification |
| Almond | E96ALM | 11 min ± 1 min | 1.9 ng/mL (ppb) | 1.0 ppm |
| Bovine Casein | E96CAS | 50 min | 1.7 ng/mL (ppb) | 0.5 ppm |
| Bovine Total Milk | E96MLK | 50 min | 5.8 ng.mL (ppb) | 1.0 ppm |
| Brazil Nut | E96BZL | 50 min | 1.3 ng/mL (ppb) | 1.0 ppm |
| Cashew | E96CHW | 50 min | 2.6 ng/mL (ppb) | 0.9 ppm |
| Coconut | E96COC | 50 min | 9.7 ng/mL (ppb) | 2.0 ppm |
| Crustacean | E96CRU | 50 min | 10.2 ng/mL (ppb) | 2.0 ppm |
| Egg White | E96EGG | 50 min | 2.1 ng/mL (ppb) | 0.5 ppm |
| Fish | E96FSH | 50 min | 2.7 ng/mL (ppb) | 1.0 ppm |
| Hazelnut | E96HZL | 50 min | 1.9 ng/mL (ppb) | 1.0 ppm |
| Macademia | E96MAC | 50 min | 1.1 ng/mL (ppb) | 0.3 ppm |
| Mollusk | E96MOL | 50 min | 7.2 ng/mL (ppb) | 1.0 ppm |
| Mustard | E96MUS | 50 min | 3.1 ng/mL (ppb) | 1.5 ppm |
| Peanut | E96PNT | 50 min | 6.7 ng.mL (ppb) | 2.0 ppm |
| Pecan | E96PEC | 50 min | 3.7 ng/mL (ppb) | 0.7 ppm |
| Pine Nut | E96PNE | 50 min | 1.3 ng/mL (ppb) | 0.5 ppm |
| Pistachio | E96PST | 50 min | 3.5 ng/mL (ppb) | 1.0 ppm |
| Sesame | E96SES | 50 min | 3.7 ng/mL (ppb) | 2.0 ppm |
| Soy | E96SOY | 50 min | 11.0 ng/mL (ppb) | 2.0 ppm |
| Walnut | E96WAL | 50 min | 6.9 ng/mL (ppb) | 2.0 ppm |
All kits include one plate of 96 breakaway wells.
Key Industries
- Biopharmaceutical and Biotechnology
- Chemical
- Cosmetics & Personal Care
- Education & Academics
- Food & Beverage
- Medical
- Pharmaceutical
- Polymers, Plastics and Rubbers
Brand
Neogen is a leader of innovative solutions that help the food and beverage industries optimize the quality of their products to enable consumer protection. At every step, Neogen provides solutions that help mitigate risk, improve operational efficiencies and impact the bottom line.