Merck – Prepare, Grow, Analyze
With industry-leading sterile filtration devices based on 60 years of membrane filtration expertise, Merck supports conventional and emerging formats for cell culture. The Merck Cell Culture Solutions portfolio features over 20,000 high-quality tools to cultivate consistency in your cell culture workflow.
Cell Preparation: Tools that you need to minimize contamination risk and maximize workflow efficiency while preparing your media for cell culture.
Cell Growth: Whatever your cell culture growth needs, we have a plate, insert or vessel that can help you obtain the optimal cell culture results.
Cell Analysis: In addition to our solid base in assay tools and products, we offer new, novel cell culture technologies and cell culture platforms for high content data collection and analysis.
Available Models
Millex® Syringe filters set the standard for reliable small volume filtration (1 mL to 200 mL).
Available in 4, 13, 25, 33, and 50 mm diameters
Minimal hold-up volume for less sample loss of small volumes and are ideal for solutions such as antibiotics and tissue culture additives. Their unsurpassed quality and consistency of results has led to the development of many sample preparation methods that specify Millex® filters.
Manufactured for reliable performance
Manufacturing occurs in a controlled environment using an automated process. Sterile devices are provided with a certificate of quality.
Faster flow rate
33 mm Millex filters have 20% more filter surface than 25 mm filters for significantly higher flow rate and throughput.
Higher operating pressure
With a maximum housing pressure of 150 psig (10 bar), solutions can be filtered faster.
Low extractables, low binding
A variety of membranes and housings ensure chemical compatibility with a range of samples and solvents.
Ordering information
- 33 mm Millex® syringe filters
- 4, 13 and 25 mm Millex® syringe filters
- 50 mm Millex® syringe filters
Stericap™ PLUS filters directly into any vacuum-rated bottle or other container with an inner neck diameter of 20 to 67 mm.
- Vacuum driven, bottle cap filter for 2 to 10 L volumes
- Fast sterilization of large volumes of tissue culture media, serum, buffers or other biological solutions
- Non-cytotoxic and non-pyrogenic devices
A handheld automated cell counter that could count the cell number in your sample accurately within 30 second using the Coulter Principle. It has been validated to use with many cell types such as NIH 3T3 Cells, Jurkat Cells, K562 cells, Human Mesenchymal Stem Cells, PBMC (Cultured), CD3+ T Cells, Human RBC (EDTA) cells and many more.
Simplify your cell analysis by using the Millicell® EZ SLIDE to culture, fix, stain and view your sample all in one device. There’s no need to remove the media chamber from the slide prior to fixing or staining. Easily remove wells without worrying about breaking slides or harming cells. Acquire data simply and quickly with Millicell® EZ SLIDEs.
Key Features
- Breakable tabs for easy well removal
- No tools required to remove wells from slide
- No adhesive used to attach wells to slide
- No leaky wells and no cross contamination
- Polypropylene wells are resistant to most fixing and staining reagents and allow for fixing and staining with wells still attached
- Slide holder protects slide from damage
- Raised corner edges on the lid facilitate stacking in the incubator
- Visualize easily without removing the lid
- Can incubate in the tray supplied with the units
Ask us about specials and custom orders with the EZ SLIDE.
The Millicell® ERS (Electrical Resistance System) reliably measures membrane potential and resistance of epithelial cells in culture [or the Trans Epithelial Electrical REsistance (TEER)]. This device qualitatively measures cell monolayer health and quantitatively measures cell confluence. An increase in TEER detected with the electronic circuit of the Millicell® ERS-2 meter and its electrode is an indication of cell monolayer health and confluence. A silver/silver chloride (Ag/AgCl) pellet on each electrode tip measures voltage. Due to the small size of the electrodes, the user can easily measure transepithelial voltage and the resistance of cells grown on microporous membranes. Please note: MERS00002 (Millicell® ERS-2) is a replacement for MERS00001 (Millicell®-ERS). This system is for research only.
The Millicell® ERS-2 system uses alternating current (AC) to make membrane resistance measurements. Using an isolated AC source has several advantages over the traditional use of direct current (DC):
- Membrane voltage and voltage electrode offset do not affect resistance measurements.
- The zero-net charge on the cells eliminates the adverse effects of DC current on the cell membrane.
- There is no electrochemical deposition of electrode metals.
- Membrane capacitance does not affect resistance readings. Once the Millicell® ERS-2 system is standardized, you can use it to quantitatively measure cell confluence.
The CellASIC® ONIX2 System incorporates extraordinary improvement to additional live cell observation and experimentation, using advanced microfluidics technology with computer automation to manipulate the culture environment under controlled conditions while allowing you to monitor the cell as they react in real time.
This second generation CellASIC® ONIX2 Microfluidic System is backed by a strong history to distinctive publications, stringent, globally certified manufacturing and quality processes, and also with local service and support.
No matter what application you’re working with, Merck has a plate developed to meet your specific needs. MultiScreen plates have a long history as a reliable tool for the advancement of drug discovery and life science research.
HTS Filter Plates
MultiScreenHTS plates are specifically developed for high-throughput use with automated work stations. Rigid sidewalls improve handling and ample surfaces are provided for barcode labels. Wells are individually sealed to prevent incubation crosstalk and the underdrain is removable for access to the filters. Filter plates are available in 96- and 384-well platforms with a broad selection of membranes and plastics.
MultiScreen® High Volume Filter Plates
Looking for a filter plate that holds 2 mL per well? The MultiScreen® High Volume filter plate can process up to 2 mL of aqueous and organic solutions, with best-in-class discrete and quantitative filtrate transfer to a custom designed 96-well MultiScreen® High Volume collection plate using the MultiScreen®HTS vacuum manifold. The MultiScreen® High Volume filter plate is fully automation-compatible and complies with ANSI Deepwell Plate standards. Choose between Durapore® (PVDF) and glass fiber high volume filter plates.
Classic Filter Plates
The original multiwell design is valuable for many applications which require immersing the filter in an assay tray. The easily removable underdrain and unique plate design are ideal for assays such as Elispot, PAMPA, cell screening (migration, invasion or chemotaxis) or for topcount detection users.
MultiScreen®HTS Vacuum Manifold
The MultiScreen®HTS Vacuum manifold was designed specifically to work with MultiScreen®HTS filter plates. The combination results in optimal filtration characteristics and seamless automation integration.
Millicell® Hanging Cell Culture Inserts are sterile, general purpose devices for the growth and differentiation of various cell types. Uniquely designed flanges suspend the insert in an off-center position within the culture plate well, facilitating pipetting by creating a larger space to one side. Millicell® hanging inserts make it possible to study both sides of the cell monolayer and are excellent tools in co-culturing and permeability assays.
Key Features
- For co-culturing and permeability assays
- Unique design allows easier basolateral access than other hanging inserts with less risk of contamination
- Available in 5 pore sizes and 3 diameters (for 24-, 12-, or 6-well plates), including a 1 µm pore size that is optically transparent for better visualization by microscopy. The inserts are easily prepared for SEM and TEM visualizing techniques, and they are compatible with cellular and/or fluorescent stains.
Our standing inserts promote excellent cell growth and are ideally suited for cell studies. Attachment or suspension cells can access media from both their apical and basolateral sides. Cell growth, structure, and function more closely mimic what occurs in vivo. In addition, Millicell® inserts make it possible to study both sides of the cell monolayer.
Key Features
- The Biopore™ (PTFE) membrane provides high viability for the superior study of three-dimensional explant structure
- Available with Biopore™ (PTFE) membrane, MF-Millipore (mixed cellulose esters) membrane, and polycarbonate membrane
For high cell viability and study of 3D explant structure, the Millicell® organotypic insert is extremely biocompatible. Explants readily adhere to its surface without an extracellular matrix. This unique membrane can sustain cell growth for as long as 40 days, with excellent trans-membrane oxygen transport.
Key Features
- The Biopore™ (PTFE) membrane provides high viability—for as long as 40 days—and excellent trans-membrane oxygen transport for the superior study of three-dimensional explant structure
- The membrane is optically clear and optimized for long-term organotypic explant maintenance
- Lower wall height of 5 mm presents a very low profile and allows them to fit inside a standard petri dish and allow for easier manipulation. Samples can be readily accessed with a microscope or electrophysiology equipment without modifying the Millicell® insert or removing the membrane from the support.
Key Industries
- Biopharmaceutical and Biotechnology
- Medical
- Pharmaceutical
Brand
At Merck, our vision is to unleash the potential of science for life. But we couldn’t do it without you. Close partnerships with our customers have been at the heart of our progress throughout our long history. They have allowed us to clearly understand your challenges. To develop high-quality solutions that are tailored to your needs. And to constantly push the bound- aries of innovation. As your reliable partner and one-stop supplier, we offer a portfolio of more than 40,000 products, and serve you from 154 locations in 67 countries around the world. So whether in your quality control lab, pilot plant or production facility, you’ll have the most suitable products, packaging and documentation to conduct your application more easily, efficiently and economically.